As shown in figure 1, inertsil ods3 has a relatively strong retentivity compared to commercially available ods columns. The inertsil 3series represents a major advance in performance over the original inertsil 2series. Microarray plate method for estimation of precipitation. This code snippet will create the output file style. Other bonded phases, such as c8, cn, phenyl and polar embedded phases, are excluded. The same high performance characteristics found in inertsil ods3 have been incorporated in the manufacture of inertsil ods3v. Inertsil ods3v gl sciences, tokyo, japan was used as an analytical column, with a 1. Inertsil ods3 100a 3m inertsil ods3 is still gl sciences most popular phase and continues to be used widely and reliably for long established methods in pharmaceutical, and contract research labs. To maximize the performance of the column, extreme care should be taken on the internal diameter of. Column performance reproducibility of inertsil ods3v.
Inertsil ods 3 series phases are based on a purer, higher surface area silica which is specially manufactured to provide maximum bonded phase coverage. The analytes were separated on inertsil ods3v, 150 mm. App id 5596 app id 5597 0 min 6 5 4 3 2 1 0 2 4 6 8 0 50. Inertsil 3series phases, including ods3, ods3v, odsp, odssprint, odsep, c83, ph3, cn3, nh2.
Retinol acetate retinyl acetate vitamin a acetate menadione vitamin k3. Hypersil stationary phases are recognized as an industry standard in hplc. Tskgel ods100v and ods100z reversed phase hplc columns. The result is a series of columns which provide excellent peak shapes using simple eluents while operating at low pressure. Spherisorb surface 15,000x magnification sem of base silica surface 15,000x magnification sem of base silica sphereclone guaranteed alternative to spherisorb comparative separations may not be representative of all applications. Inertsil column care hplc columns in india, chromatography in. Inertsil ods 3 users can, therefore, readily change to the new, validated inertsil ods 3v columns with complete confidence. Gl sciences inertsil ods4 offers improved peak shape, enhanced sensitivity, high resolution, fast equilibration and is. La123 anion surfactants technical support gl sciences. Hplc column selection by manufacturer dikma technologies. Inertsil ods3v gl sciences 502030142 502030144 502030145 502001801 502001802. Inertsil odsp is available in 5 micron particle size in column configurations ranging from capillary to 50mm id preparative sizes. Therefore, inertsil ods 4 can be used at ease under 100% aqueous eluents. Inertsil ods3v series product data 1020 gl sciences.
Ods pdf example this code snippet will create the output file output. Proven as an effective analytical tool, classical hyp. Inertsil ods3 provides virtually perfect peak shape for most bases and acids. Inertsil ods3v is a superior ods column offering the same chromatographic characteristics as inertsil ods3. Gl sciences inc 1210l150w046 inertsil ods2, 5 micron, 150 x 4. Inertsil ods3v columns are also available in 3columnsets packed with your choice of 3 different silica batches or a single silica batch to assist in reproducibility. Only silica based c18 bonded phases are evaluated in this guide. Comparison guide to c18 reversed phase hplc columns. For further information on this new phase or to request a free sample column visit. Inertsil ods3 lichrospher rp18 ymc jsphere ods h80 nucleosil c18 kromasil c18 develosil odsmg partisil ods2 lichrosorb rp18 symmetry c18 inertsil ods develosil odsug purospher rp18e ymc pro c18 prodigy ods3 zorbax ods nucleosil c18hd sunfire c18 ymc ods am ymc ods a inertsil ods2 cosmosil c18ar prodigy ods2 tsk ods80tm ace c18 luna. Special application hplc columns other applications.
Method development and validation of rp hplc method for. Inertsil 3series phases, including ods 3, ods 3v, ods p, ods sprint, ods ep, c83, ph3, cn3, nh2. Take note of the solvent contained in the column during shipping solvent. What is the chemistry behind preparing a c18 column. Hplc column technical guide introduction specifications shipping solvent mobile phase sample diluentinjection solvent column cleaning and storage tips to increase hplc column lifetime benefits of scaling down column internal diameter size. Convert to 3fr arw bmp cr2 crw dcr dng dwg dxf emf eps erf gif heic ico jpg jpeg mdi mrw nef odg orf pcx pef png ppm psd raf svg tga tif tiff wbmp webp wmf x3f xcf csv djvu doc docx eml html html4. Inertsil ods3 users can, therefore, readily change to the new, validated inertsil ods3v columns with complete confidence. Tskgel ods80ts qa lcolumn inertsil ods3v capcellpak ug120 capcellpak acr capcellpak mg capcellpak mg ii ymcpack pro c18 rs luna c18 develosil odsug5 develosil.
Gl sciences inc inertsil ods2 5 micron 150 inertsil ods2 5. The easy and simple, twostep process requires you to upload the file, then click on pdf as the required target file format and click on convert. Luliconazole and methyl paraben has showed 98102% recovery. To maximize column life, operate at pressures within 50mpa approx. Hopefully, this infor mation will help you find the right column for your application quicker. Chromatographic properties 4 41 column efficiency 4 42 retention 4 43 column pressure drop 4 44 h u curve van deemter curve 4. Chromatographic separation was performed on inertsil ods 3v, 4.
The c18 columnscartridges in my lab are prepared using aqueous solvents, then organic solvents, then back to aqueous solvents usually a combination of water, methanol and acetonitrile. The original 2series inertsil phases, including silica, c4, c8, ods 2 and phenyl, created the high purity silica revolution in the late 1980s. A manufactures validation certificate which displays the analytical data of the lot is attached to each column. Gl sciences inertsil ods 4 offers improved peak shape, enhanced sensitivity, high resolution, fast equilibration and is compatible with 100% aqueous mobile phases. Inertsil hplc packings are subject to a rigorous array of qc tests in a iso9001 compliant facility, with special emphasis on reagent purity, raw material traceability, and consistency in raw materials and finished. Filled with stationary phase compositions or resins, the prepacked options reduce the preparing time required. All standard geometries available most from stock priced competitively. Inertsil ods4 minimizes the dewetting phenomenon while maintaining the same retention characteristics to that of common ods columns. Aqua c18 ace aq ultra aqueousc18 ymcpack odsaq aqua c18 zorbax sbaq syncronis aq c18. Phenomenex uhplc, hplc, spe, gc leader in analytical. The method has been linear for both compounds at 50150% range with r2 0.
Therefore, inertsil ods4 can be used at ease under 100% aqueous eluents. Convert ods to pdf online and free this page also contains information on the ods and pdf file extensions. Thermo scientific hypersil ods2 c18 hplc columns are an alternative to older column brands commonly referenced in validated methods. Hypersil ods c18 hplc columns, thermo scientific vwr. Pdf the estimation of maraviroc in tablet dosage form by. Inertsil ods3v is a highly efficient, validated hplc column, developed to provide optimum separation for a wide range of acidic and basic compounds. Development of simple hplcuv with a columnswitching method. Detection wavelength for luliconazole was 294 nm and for methyl paraben was 254nm. Pdf the estimation of maraviroc in tablet dosage form by rp. The ods pdf statement opens the pdf destination and creates pdf output.
Pdf file format, developed by adobe systems, represents in electronic mode all the elements of a printed document such as text, photos, links, scales, graphs and interactive elements. Inertsil ordering information 3um ordering information 5um manufactured by gl sciences based in japan 3um od3, 5um ods3, c83, phenyl3 and cn3 phases. Inertsil ods3v specifically qualified hplc columns for glpgmp. This guide does not identify an overall best column.
Tskgel ods 100v and ods 100z reversed phase hplc columns table of contents 1. Analytical method of cel was developed on a shimadzu hplc system using inertsil ods3v column 4. Discount price princetonspher, inertsil hplc columns. The performance is checked for individual columns by measuring the number of theoretical plates and. Dodecyl sulfate sodium lauryl sulfate sds ntridecyl sulfate sodium salt. Inertsil hplc column catalog inertsil hplc column catalog. Inertsil ods3 2um instruction for use when using an ultra high pressure specification instrument, please be careful with the fitting type. The original 2series inertsil phases, including silica, c4, c8, ods2 and phenyl, created the. The vertical pieces are able to withstand extreme temperatures.
Hypersil ods2 c18 columns thermo fisher scientific. Inertsil ods 5 100 350 14 yes no inertsil ods3 5 100 450 15 yes yes inertsil ods2 5 150 320 18. New hplc column in box with all documentation 2006gl sciences inertsil odssp hplc column, 4. Inertsil hplc packings are subject to a rigorous array of qc tests in a iso9001 compliant facility, with special emphasis on reagent purity, raw material traceability, and consistency in raw materials and finished products. Each inertsil ods3v is delivered with a manufacturers validation certificate showing the detailed results of every qa and qc step in manufacturing. Gl sciences inc inertsil ods2 5 micron 150 inertsil ods2. The present study also introduced a column selection unit hv208001 jasco, tokyo, japan, as a column. Inertsil ods p is available in 5 micron particle size in column configurations ranging from capillary to 50mm id preparative sizes. Because no style definition is specified, the default style, styles.
Furthermore, the packing material that achieves a stable analysis using 100% aqueous eluents provides another. The original 2series inertsil phases, including silica, c4, c8, ods2 and phenyl, created the high purity silica revolution in the late 1980s. Gl sciences have announced the launch of their latest ultrainert ods phase, inertsil ods4. You can view this document in free acrobat reader, navigate through the page or.
This phase is ideal for acidic, basic, neutral and chelating compounds. With its high reproducibility, inertsil ods3v is available for validation. Phenomenex is a global manufacturer of uhplc, hplc columns, gc columns, sample preparation products and chromatography accessories and consumables. Method development and validation of rp hplc method for assay.
The same high performance characteristics found in inertsil ods 3 have been incorporated in the manufacture of inertsil ods 3v. The use of inertsil ods3v columns provides an extra measure of assurance of consistent performance from column to column and batch to batch. The detection was carried out by using pda detector at 275 nm. Gl sciences inc inertsil ods2 5 micron 150 manufacturer. Ods 3 m, 5 m 100 a inertsil 3series phases are based on a purer, higher surface area silica which is specially manufactured to provide maximum bonded phase coverage. Inertsil ods3 is ideally modified with octadecyl groups and endcapped. Inertsil ods3 series phases are based on a purer, higher surface area silica which is specially manufactured to provide maximum bonded phase coverage. Inertsil inods3150dea 0 gbp inods3150d 5547593 5547596 502001900 5547594 inods3250d 502001921 5547588 5547595 5547585 5547587 502005044 5547586 inods2150a. Stabilityindicating hplc method for determination of. Development of simple hplcuv with a columnswitching. Ods80a was originally introduced to provide a higher surface area alternative to ods2, but with the same inertness and basedeactivated properties. Inertsil ods 4 minimizes the dewetting phenomenon while maintaining the same retention characteristics to that of common ods columns. Classical hypersil stationary phases have global recognition as an industry standard in hplc, providing an effective analytical toolchromatography columns separate molecules within mixtures during gas or liquid methods.
Inertsil ods3v inertsil ods3 validated column l1 3, 5 10 450 inertsil odssp weak retentivity, for hydrophobic compounds l1 3, 5 10 450 inertsil odsp high steric selectivity l1 3, 5 10 450. Ods to pdf convert your ods to pdf for free online. Tskgel ods100v and ods100z reversed phase hplc columns table of contents 1. Ods 80a was originally introduced to provide a higher surface area alternative to ods 2, but with the same inertness and basedeactivated properties.
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